What is cyanol ff?

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xy·lene cy·a·nol FF [C.I. 43535] an acidic triphenylmethane dye used for histochemical staining of hemoglobin peroxidase and as a tracking dye for DNA sequencing in electrophoresis.

What is xylene cyanol?

Visit Product Comparison Guide Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.

What is the concentration of xylene cyanol in bromophenol?

Bromophenol blue and orange G can also be used for this purpose. Once mixed with the sample, the concentration of xylene cyanol is typically about 0.005% to 0.03%.

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What is the migration speed of xylene cyanol?

Migration speed. In 1% agarose gels, xylene cyanol migrates at about the same rate as a 4 to 5 kilobase pair DNA fragment, although this depends on the buffer used. Xylene cyanol on a 6% polyacrylamide gel migrates at the speed of a 140 base pair DNA fragment.

What is the concentration of xylene cyanol in agarose?

Once mixed with the sample, the concentration of xylene cyanol is typically about 0.005% to 0.03%. In 1% agarose gels, xylene cyanol migrates at about the same rate as a 4 to 5 kilobase pair DNA fragment, although this depends on the buffer used. Xylene cyanol on a 6% polyacrylamide gel migrates at the speed of a 140 base pair DNA fragment.


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More about What is cyanol ff?


1. Xylene cyanole FF | C25H27N2NaO6S2 – PubChem

Xylene Cyanol FF. Xylene cyanole FF. 2650-17-1. MFCD00019481. Acid blue 147. 1,3-Benzenedisulfonic acid, 4-[[4-(ethylamino)-3-methylphenyl][4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-, sodium salt (1:1) C25H27N2O6S2.Na. DTXSID20883877. AMY22431

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2. Xylene Cyanol FF BioReagent, for molecular biology 2650-17-1

Xylene Cyanol FF (Acid blue 147 ); BioReagent, for molecular biology; Suitable for use with agarose or polyacrylamide gel electrophoresis (PAGE); Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis; Xylene Cyanol FF …

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4. Xylene cyanol blue FF, FF stands for – ResearchGate

I found xylene cyanol FF in a dyestuff publication from 1921, and the FF designation was in use in 1905. The reference below says that it means superfine (as in F for fine, FF for superfine), but …

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5. Xylene cyanol FF, 75%, Thermo Scientific | Fisher Scientific

Xylene cyanol FF, ∽75%, Thermo Scientific™ Click to view available options Quantity: 10g 50g Packaging: Glass bottle CAS: 2650-17-1: Molecular Formula: C 2 5 H 2 7 N 2 NaO 6 S 2: MDL Number: MFCD00040657: Synonym: Acid blue 147 monosidium salt, C.I. 42135 …

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6. Xylene Cyanol – an overview | ScienceDirect Topics

Xylene cyanol FF agarose gel loading buffer, for example, 6 × Orange DNA Loading Dye (Thermo Scientific) [if possible avoid loading buffers with Bromophenol blue, since it will stain the area of the agarose gel which is of interest for EMOTE].. Molecular marker for DNA sizes (for example, GeneRuler 1 kb Plus DNA Ladder from Thermo Scientific). …

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7. SAFETY DATA SHEET

Product Name Xylene cyanol FF. Cat No. : AC422690000; AC422690050; AC422690250; AC422690500; AC422691000; AC422692500; AC422695000. CAS No4463-44-9. SynonymsC.I. 43535. Recommended UseLaboratory chemicals. Uses advised againstFood, drug, pesticide or biocidal product use. Details of the supplier of the safety data sheet.

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9. Preparation of 6X DNA Gel loading buffer (Bromophenol blue …

Depending on your requirements, you can change the concentration of tracking dyes (0.03% to 0.50% (w/v) for bromophenol blue and xylene cyanol FF and 0.15% – 0.50% (w/v) for Orange G). The high concentration provides a good contrast color, which is easy to monitor upon electrophoresis progression but also interfere in the analysis of …

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10. Xylene cyanol FF, tracking dye for nucleic acids (CAS 2650 …

Biological description. Tracking dye commonly used for nucleic acids. Used in both agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.

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